segunda-feira, 20 de outubro de 2014

Um bate-papo ao cair da noite - V

 

Esta semana li um banner em um importante website dos Estados Unidos, mais ou menos isto, não me lembro exatamente. “O que o Brasil precisa para mudar”…. Como isso é um tema que diz respeito à todos os países do mundo, ou quase todos, eu acho que somos muito queridos pelos nossos irmãos de outros países, e eu como brasileiro nato, fico até emocionado. (Maria, por favor, traga-me aquele lencinho de enxugar lágrimas…)

Bem, como mencionei acima, poucos países no mundo, estão tranquilos do ponto de vista econômico e social.  A economia mundial, e os valores morais da sociedade, hoje estão intimamente ligados. Um país avança em todos os sentidos quando o Mundo dá sinais de melhoria. O mundo é uma imensa máquina, e os países são as engrenagens dela. Qualquer problema numa engrenagem importante da máquina, e tudo fica meio emperrado.

Interessa muito ao mundo que o Brasil, (um país importante no cenário internacional, o 5º em área territorial, um dos maiores em população, GDP entre os maiores do mundo)… deslanche econômicamente, afinal somos um páis-alvo para as grandes empresas dos países (quase) desenvolvidos, e temos um grande contingente de consumidores, ávidos consumidores. Mas a máquina mundial está no momento meio emperrada….e isso nos afeta. Alguma ou algumas engrenagens estão precisando de ajustes, lubrificação….e isso nos afeta…e afeta a todos. Nada melhor do que um Simpósio Internacional para discutir isso… Parece que “Simpósio” não é o termo correto…Talvez “Conferência”, Reunião….

Mas, precisamos de um suporte mais efetivo dessas potências econômicas…. que pode ser realizado de várias maneiras…. (Transferência de tecnologias, por exemplo….) Ou aumento nas importações de produtos brasileiros…..

O que o Brasil precisa para mudar… Precisamos que o mundo mude junto conosco…. 

José S de Melo

Something about Belgium

 

Belgium  Dutch: België; French: Belgique; German: Belgien), officially the Kingdom of Belgium, is a federal monarchy in Western Europe. It is a founding member of the European Union and hosts the EU's headquarters as well as those of several other major international organisations such as NATO.[nb 1] Belgium covers an area of 30,528 square kilometres (11,787 sq mi) and has a population of about 11 million people.

Straddling the cultural boundary between Germanic and Latin Europe, Belgium is home to two main linguistic groups: the Dutch-speaking, mostly Flemish community, which constitutes about 59% of the population, and the French-speaking, mostly Walloon population and Brussels inhabitants, which comprises 41% of all Belgians. Additionally, there is a small group of German-speakers who are officially recognized.

Belgium's two largest regions are the Dutch-speaking region of Flanders in the north and the French-speaking southern region of Wallonia. The Brussels-Capital Region, officially bilingual, is a mostly French-speaking enclave within the Flemish Region. A German-speaking Community exists in eastern Wallonia. Belgium's linguistic diversity and related political conflicts are reflected in its political history and complex system of government.

Historically, Belgium, the Netherlands, and Luxembourg were known as the Low Countries; it once covered a somewhat larger area than the current Benelux group of states. The region was called Belgica in Latin, after the Roman province of Gallia Belgica, which covered more or less the same area. From the end of the Middle Ages until the 17th century, the area of Belgium was a prosperous and cosmopolitan centre of commerce and culture. From the 16th century until the Belgian Revolution in 1830, when Belgium seceded from the Netherlands, the area of Belgium served as the battleground between many European powers, causing it to be dubbed the "Battlefield of Europe," a reputation strengthened by both World Wars.

Upon its independence, Belgium participated in the Industrial Revolution and, during the course of the 20th century, possessed a number of colonies in Africa.The second half of the 20th century was marked by rising tensions between the Dutch-speaking and the French-speaking citizens fueled by differences in language and the unequal economic development of Flanders and Wallonia. This continuing antagonism has led to several far-reaching reforms, resulting in a transition from a unitary to a federal arrangement during the period from 1970 to 1993. Despite the reforms, tensions between the groups remain; the formation of a coalition government took 18 months following the June 2010 federal election.

 

Bruegge_huidenvettersplein

Bruegge Huidenvettersplein

 

Liege_View

Liege View

Hubble finds extremely distant galaxy through cosmic magnifying glass

 


The heart of the mammoth galaxy cluster Abell 2744, also known as Pandora's Cluster, is shown in this Hubble Space Telescope image. The cluster is so massive that its powerful gravity bends the light from galaxies far behind it, making background objects appear larger and brighter in a phenomenon called gravitational lensing. These powerful lenses allow astronomers to find many dim, distant structures that otherwise might be too faint to see. The small white boxes, labeled "a," "b," and "c," mark multiple images from the same background galaxy, one of the farthest, faintest, and smallest galaxies ever seen. The diminutive object is estimated to be over 13 billion light-years away. Enlarged views of the multiple images are shown in the insets at right.

Peering through a giant cosmic magnifying glass, NASA's Hubble Space Telescope has spotted one of the farthest, faintest, and smallest galaxies ever seen. The diminutive object is estimated to be over 13 billion light-years away.

This new detection is considered one of the most reliable distance measurements of a galaxy that existed in the early universe, said the Hubble researchers. They used two independent methods to estimate its distance.

The galaxy appears as a tiny blob that is only a small fraction of the size of our Milky Way galaxy. But it offers a peek back into a time when the universe was only about 500 million years old, roughly 3 percent of its current age of 13.7 billion years. Astronomers have uncovered about 10 other galaxy candidates at this early era. But this newly found galaxy is significantly smaller and fainter than most of those other remote objects detected to date.

"This object is a unique example of what is suspected to be an abundant, underlying population of extremely small and faint galaxies at about 500 million years after the big bang," explained study leader Adi Zitrin of the California Institute of Technology in Pasadena. "The discovery is telling us that galaxies as faint as this one exist, and we should continue looking for them and even fainter objects so that we can understand how galaxies and the universe have evolved over time."

The galaxy was detected as part of the Frontier Fields program, an ambitious three-year effort, begun in 2013, that teams Hubble with NASA's other Great Observatories -- the Spitzer Space Telescope and the Chandra X-ray Observatory -- to probe the early universe by studying large galaxy clusters. These clusters are so massive that their gravity deflects light passing through them, magnifying, brightening, and distorting background objects in a phenomenon called gravitational lensing. These powerful lenses allow astronomers to find many dim, distant structures that otherwise might be too faint to see.

In this new discovery, the lensing power of the mammoth galaxy cluster Abell 2744, nicknamed Pandora's Cluster, produced three magnified images of the same galaxy. Each magnified image makes the galaxy appear as much as 10 times larger and brighter than it would look without the intervening lens.

An analysis of the distant galaxy shows that it measures merely 850 light-years across, 500 times smaller than the Milky Way, and is estimated to have a mass of only 40 million suns. The galaxy's star formation rate is about one star every three years (one-third the star formation rate in the Milky Way). Although this may seem low, Zitrin said that given its small size and low mass, the tiny galaxy is in fact rapidly evolving and efficiently forming stars.

"Galaxies such as this one are probably small clumps of matter that are starting to form stars and shine light, but they don't have a defined structure yet," Zitrin said. "Therefore, it's possible that we only see one bright clump magnified due to the lensing, and this is one possibility as to why it is smaller than typical field galaxies of that time." Zitrin's team spotted the galaxy's gravitationally multiplied images using near-infrared and visible-light photos of the galaxy cluster taken by Hubble's Wide Field Camera 3 and Advanced Camera for Surveys. But at first they didn't know how far away it was from Earth.

Normally, astronomers use spectroscopy to determine an object's distance. The farther away a galaxy, the more its light has been stretched by the universe's expansion. Astronomers can precisely measure this effect through spectroscopy, which characterizes an object's light.

But the gravitationally lensed galaxy and other objects found at this early epoch are too far away and too dim for astronomers to use spectroscopy. Astronomers instead analyze an object's color to estimate its distance. The universe's expansion reddens an object's color in predictable ways, which scientists can measure.

Members of Zitrin's team not only performed the color-analysis technique, but they also took advantage of the multiple images produced by the gravitational lens to independently confirm their distance estimate. The astronomers measured the angular separation between the three magnified images of the galaxy in the Hubble photos. The greater the angular separation due to lensing, the farther away the object is from Earth. To test this concept, the astronomers compared the three magnified images with the locations of several other multiply imaged objects lensed by Abell 2744 that are not as far behind the cluster. The angular distance between the magnified images of the closer galaxies was smaller.

"These measurements imply that, given the large angular separation between the three images of our background galaxy, the object must lie very far away," Zitrin explained. "It also matches the distance estimate we calculated, based on the color-analysis technique. So we are about 95 percent confident that this object is at a remote distance, at redshift 10 (a measure of the stretching of space since the big bang). The lensing takes away any doubt that this might be a heavily reddened, nearby object masquerading as a far more distant object."

Astronomers have long debated whether such early galaxies could have provided enough radiation to warm the hydrogen that cooled soon after the big bang. This process, called "reionization," is thought to have occurred 200 million to 1 billion years after the birth of the universe. Reionization made the universe transparent to light, allowing astronomers to look far back into time without running into a "fog" of cold hydrogen.

"We tend to assume that galaxies ionized the universe with their ultraviolet light," Zitrin said. "But we do not see enough galaxies or light that could do that. So we need to look at fainter and fainter galaxies, and the Frontier Fields and galaxy cluster lensing can help us achieve this goal."


Story Source:

The above story is based on materials provided by Space Telescope Science Institute (STScI). Note: Materials may be edited for content and length.


Journal Reference:

  1. Adi Zitrin, Wei Zheng, Tom Broadhurst, John Moustakas, Daniel Lam, Xinwen Shu, Xingxing Huang, Jose M. Diego, Holland Ford, Jeremy Lim, Franz E. Bauer, Leopoldo Infante, Daniel D. Kelson, Alberto Molino. A GEOMETRICALLY SUPPORTEDz∼ 10 CANDIDATE MULTIPLY IMAGED BY THE HUBBLE FRONTIER FIELDS CLUSTER A2744. The Astrophysical Journal, 2014; 793 (1): L12 DOI: 10.1088/2041-8205/793/1/L12

 

The first Indian satellite

 

Snap 2014-10-19 at 04.40.26

Interesting

 

 

Snap 2014-10-19 at 04.52.57

Será que ela perdoou?

Snap 2014-10-19 at 04.51.14

Vejam como as mamães sofrem.

Snap 2014-10-19 at 04.49.47

Which couple has won the dance marathon?

Snap 2014-10-19 at 04.48.12

The “tiny” 5MB HDisk (enough to store a MP3 music)

Snap 2014-10-19 at 04.33.17

Fidel Castro and Che Guevara

Snap 2014-10-19 at 04.35.22

Coca Cola in France for the first time.

Snap 2014-10-19 at 04.32.35

A London Pub

 

Snap 2014-10-19 at 04.24.23

King George V

 

Snap 2014-10-19 at 04.25.57

It seems the beggar didn’t get anything.

Harley-Davidson Motor Company

 

Snap 2014-10-19 at 04.42.34

Smartphone approach for examining progression of diabetic eye disease offers comparable results to traditional method

 


A smartphone-based tool may be an effective alternative to traditional ophthalmic imaging equipment in evaluating and grading severity of a diabetic eye disease, according to a study released today at AAO 2014, the American Academy of Ophthalmology's 118th annual meeting. The results of the research indicate the lower-cost method could be useful for bringing the service to patients in isolated or underserved communities.

Approximately 7.7 million Americans have diabetic retinopathy, which is caused by elevated blood glucose (sugar) levels and can lead to vision loss and blindness.The traditional method for monitoring the progression of the disease is through retinal slit-lamp biomicroscopy, which enables ophthalmologists to look at the back of the eye's interior. This kind of examination requires a large piece of specialized equipment found only in clinical settings, posing a significant challenge for monitoring patients living in rural or low-resource communities.

In order to find a solution for addressing this challenge, researchers from the University of Brescia, University of Molise and "Federico II" University of Naples, Italy, developed a small optical adapter called D-Eye which could attach magnetically to an iPhone® 5, creating a smartphone ophthalmoscope. They then used the iPhone ophthalmoscope as well as a slit-lamp biomicroscope to perform dilated retinal digital imaging on 120 patients with diabetes who were scheduled to have a routine dilated eye exam. After comparing the results of the smartphone method to the traditional one, an exact agreement between the two methods was found in 85 percent of the eyes and an agreement within one step (or grade of disease progression) was found in 96.7 percent of the eyes. In most of the one- and two-step disagreements, the severity level was graded higher by biomicroscopy grading.

In the smartphone ophthalmoscopy results, nine eyes were not gradable due to small pupil or cataract. In the biomicroscopy results, the number of not gradable images was four. Therefore, while biomicroscopy is still found to be the more accurate method for grading diabetic retinopathy, researchers believe smartphone ophthalmoscopy shows great potential for use in rural or remote communities who would normally receive little to no testing at all.

"Using the iPhone method is thousands of dollars cheaper than using traditional equipment," said lead researcher Andrea Russo, M.D. "The affordability of this option could make it much easier to bring eye care to non-hospital remote or rural settings, which often lack ophthalmic medical personnel."


Story Source:

The above story is based on materials provided by American Academy of Ophthalmology (AAO). Note: Materials may be edited for content and length.


 

Nixie quadcopter concept aims to take selfies to the next level

 

Nixie is a wearable drone designed for autonomous seflies

Nixie is a wearable drone designed for autonomous seflies

Image Gallery (3 images)

Nixie is a camera-equipped quadcopter designed to follow the user around taking pictures and videos on command, while conveniently wrapping around the wrist to form a bracelet when not in use.

The selfie is officially an international phenomenon. With some companies boasting about how their smartphones pack "perfect for selfie" front-facing shooters, and others going as far as releasing bizarre cameras designed specifically for the purpose – whether you like it or not, it’s here to stay.

Enter Nixie, the tiny quadcopter that aims to evolve the concept by taking the camera out of your hands and into the air. The idea is fairly straightforward – take a tiny drone, fit it with a swiveling camera and have it follow the user around capturing photos or video. In that way, it's quite similar to the proposed MeCam drone. What makes it more interesting (and a little whacky) is the ability to fold the quadcopter's limbs to turn into a wearable bracelet.

The Nixie drone in its prototype form

At present, Nixie is still a concept, with the company yet to produce a fully working version (you can see it in prototype form above). The product is one of 10 finalists in Intel’s Make it Wearable (MIW) challenge, the winner of which will receive US$500,000 to help turn their concept into a reality.

If it comes to fruition, then the final product will pair with a smartphone app, taking off on command, hovering a little way away to put the user in the frame before snapping a photo or video. Flight will be fully automatic, without the need for manual input, and the drone will return to the user when finished.

With the Nixie drone still being a concept, details such as battery life and specs are yet to be confirmed, but we do know that users will be able to switch it into a specific mode for selfies, panoramas or continuous movies.

 

Source: Nixie

 

These 29 Clever Drawings Will Make You Question Everything Wrong With The World

 

The Mind Unleashed

on 27 May, 2014 at 00:42

http://themindunleashed.org/wp-content/uploads/2014/05/imageee.jpg

Polish artist Pawel Kuczynski has worked in satirical illustration since 2004, specializing in thought-provoking images that make his audience question their everyday lives. His subjects deal with everything from social media to politics to poverty, and all have a very distinct message if you look closely enough

Confessions

Confessions

 

 

 

 

Even if you don’t agree with the messages behind some of these illustrations, it’s impossible not to appreciate the creativity involved in them. Maybe we really do need to start paying more attention to the things we accept as part of our daily reality.

Snap 2014-10-20 at 10.42.44

A New Solution That Stops Snoring and Lets You Sleep

 

A New Solution That Stops Snoring and Lets You Sleep

If you’re like most US people you probably don’t get eight hours sleep each night.

But, if you also constantly feel exhausted, experience headaches for no obvious reason or have high blood pressure, you could have a more serious problem.

That’s because these can all be the result of snoring—which is, in turn, the most common symptom of a potentially serious health problem—obstructive sleep apnea (OSA).

While most people think of snoring as a minor annoyance, research shows it can be hazardous to your health.  That’s because for over 18 million Americans it’s related to obstructive sleep apnea (OSA). People who suffer from OSA repeatedly and unknowingly stop breathing during the night due to a complete or partial obstruction of their airway.  It occurs when the jaw, throat, and tongue muscles relax, blocking the airway used to breathe.  The resulting lack of oxygen can last for a minute or longer, and occur hundreds of times each night. 
Thankfully, most people wake when a complete or partial obstruction occurs, but it can leave you feeling completely exhausted.  OSA has also been linked to a host of health problems including:

  • Acid reflux
  • Frequent nighttime urination
  • Memory loss
  • Stroke
  • Depression
  • Diabetes
  • Heart attack

People over 35 are at higher risk.

OSA can be expensive to diagnosis and treat, and is not always covered by insurance.  A sleep clinic will require an overnight visit (up to $5,000).  Doctors then analyze the data and prescribe one of several treatments.  These may require you to wear uncomfortable CPAP devices that force air through your nose and mouth while you sleep to keep your airways open, and may even include painful surgery.

Fortunately, there is now a comfortable, far less costly and invasive treatment option available.  A recent case study published by Eastern Virginia Medical School's Division of Sleep Medicine in the Journal of Clinical Sleep Medicine concludes that wearing a simple chinstrap while you sleep can be an effective treatment for OSA.

The chin strap, which is now available from a company called MySnoringSolution, works by supporting the lower jaw and tongue, preventing obstruction of the airway.  It’s made from a high-tech, lightweight, and super-comfortable material.  Thousands of people have used the MySnoringSolution chinstrap to help relieve their snoring symptoms, and they report better sleeping, and better health overall because of it.

MySnoringSolutions

 

Major breakthrough could help detoxify pollutants

 


Smokestacks (stock image). "As well as combatting the toxicity and longevity of pollutants we're also confident that our findings can help to develop a better method for screening environmental or food samples," one scientists noted.

Scientists at The University of Manchester hope a major breakthrough could lead to more effective methods for detoxifying dangerous pollutants like PCBs and dioxins. The result is a culmination of 15 years of research and has been published in Nature. It details how certain organisms manage to lower the toxicity of pollutants.

The team at the Manchester Institute of Biotechnology were investigating how some natural organisms manage to lower the level of toxicity and shorten the life span of several notorious pollutants.

Professor David Leys explains the research: "We already know that some of the most toxic pollutants contain halogen atoms and that most biological systems simply don't know how to deal with these molecules. However, there are some organisms that can remove these halogen atoms using vitamin B12. Our research has identified that they use vitamin B12 in a very different way to how we currently understand it."

He continues: "Detailing how this novel process of detoxification works means that we are now in a position to look at replicating it. We hope that ultimately new ways of combating some of the world's biggest toxins can now be developed more quickly and efficiently."

It's taken Professor Leys 15 years of research to reach this breakthrough, made possible by a dedicated European Research Council (ERC) grant. The main difficulty has been in growing enough of the natural organisms to be able to study how they detoxify the pollutants. The team at the MIB were finally able to obtain key proteins through genetic modification of other, faster growing organisms. They then used X-ray crystallography to study in 3D how halogen removal is achieved.

The main drive behind this research has been to look at ways of combatting the dozens of very harmful molecules that have been released into the environment. Many have been directly expelled by pollutants or from burning household waste. As the concentration of these molecules has increased over time their presence poses more of a threat to the environment and humanity. Some measures have already been taken to limit the production of pollutants, for example PCBs were banned in the United States in the 1970s and worldwide in 2001.

Professor Leys says: "As well as combatting the toxicity and longevity of pollutants we're also confident that our findings can help to develop a better method for screening environmental or food samples."


Story Source:

The above story is based on materials provided by Manchester University. Note: Materials may be edited for content and length.


Journal Reference:

  1. Karl A. P. Payne, Carolina P. Quezada, Karl Fisher, Mark S. Dunstan, Fraser A. Collins, Hanno Sjuts, Colin Levy, Sam Hay, Stephen E. J. Rigby & David Leys. Reductive dehalogenase structure suggests a mechanism for B12-dependent dehalogenation. Nature, October 2014 DOI: 10.1038/nature13901

 

Crystallizing the DNA nanotechnology dream

 


DNA has garnered attention for its potential as a programmable material platform that could spawn entire new and revolutionary nanodevices in computer science, microscopy, biology, and more. Researchers have been working to master the ability to coax DNA molecules to self assemble into the precise shapes and sizes needed in order to fully realize these nanotechnology dreams.

For the last 20 years, scientists have tried to design large DNA crystals with precisely prescribed depth and complex features -- a design quest just fulfilled by a team at Harvard's Wyss Institute for Biologically Inspired Engineering. The team built 32 DNA crystals with precisely-defined depth and an assortment of sophisticated three-dimensional (3D) features, an advance reported in Nature Chemistry.

The team used their "DNA-brick self-assembly" method, which was first unveiled in a 2012 Science publication when they created more than 100 3D complex nanostructures about the size of viruses. The newly-achieved periodic crystal structures are more than 1000 times larger than those discrete DNA brick structures, sizing up closer to a speck of dust, which is actually quite large in the world of DNA nanotechnology.

"We are very pleased that our DNA brick approach has solved this challenge," said senior author and Wyss Institute Core Faculty member Peng Yin, Ph.D., who is also an Associate Professor of Systems Biology at Harvard Medical School, "and we were actually surprised by how well it works."

Scientists have struggled to crystallize complex 3D DNA nanostructures using more conventional self-assembly methods. The risk of error tends to increase with the complexity of the structural repeating units and the size of the DNA crystal to be assembled.

The DNA brick method uses short, synthetic strands of DNA that work like interlocking Lego® bricks to build complex structures. Structures are first designed using a computer model of a molecular cube, which becomes a master canvas. Each brick is added or removed independently from the 3D master canvas to arrive at the desired shape -- and then the design is put into action: the DNA strands that would match up to achieve the desired structure are mixed together and self assemble to achieve the designed crystal structures.

"Therein lies the key distinguishing feature of our design strategy -- its modularity," said co-lead author Yonggang Ke, Ph.D., formerly a Wyss Institute Postdoctoral Fellow and now an assistant professor at the Georgia Institute of Technology and Emory University. "The ability to simply add or remove pieces from the master canvas makes it easy to create virtually any design."

The modularity also makes it relatively easy to precisely define the crystal depth. "This is the first time anyone has demonstrated the ability to rationally design crystal depth with nanometer precision, up to 80 nm in this study," Ke said. In contrast, previous two-dimensional DNA lattices are typically single-layer structures with only 2 nm depth.

"DNA crystals are attractive for nanotechnology applications because they are comprised of repeating structural units that provide an ideal template for scalable design features," said co-lead author graduate student Luvena Ong.

Furthermore, as part of this study the team demonstrated the ability to position gold nanoparticles into prescribed 2D architectures less than two nanometers apart from each other along the crystal structure -- a critical feature for future quantum devices and a significant technical advance for their scalable production, said co-lead author Wei Sun, Ph.D., Wyss Institute Postdoctoral Fellow.

"My preconceived notions of the limitations of DNA have been consistently shattered by our new advances in DNA nanotechnology," said William Shih, Ph.D., who is co-author of the study and a Wyss Institute Founding Core Faculty member, as well as Associate Professor in the Department of Biological Chemistry and Molecular Pharmacology at Harvard Medical School and the Department of Cancer Biology at the Dana-Farber Cancer Institute. "DNA nanotechnology now makes it possible for us to assemble, in a programmable way, prescribed structures rivaling the complexity of many molecular machines we see in Nature."

"Peng's team is using the DNA-brick self-assembly method to build the foundation for the new landscape of DNA nanotechnology at an impressive pace," said Wyss Institute Founding Director Don Ingber, M.D., Ph.D. "What have been mere visions of how the DNA molecule could be used to advance everything from the semiconductor industry to biophysics are fast becoming realities."


Story Source:

The above story is based on materials provided by Wyss Institute for Biologically Inspired Engineering at Harvard. Note: Materials may be edited for content and length.


Journal Reference:

  1. Yonggang Ke, Luvena L. Ong, Wei Sun, Jie Song, Mingdong Dong, William M. Shih, Peng Yin. DNA brick crystals with prescribed depths. Nature Chemistry, 2014; DOI: 10.1038/nchem.2083

 

Cellular self-destruct program has deep roots throughout evolution

 


In what seems like a counter-intuitive move against survival, within animals, some cells are fated to die from the triggering of an elaborate cell death program, known as apoptosis. Now, Sakamaki et. al., have honed in on understanding the evolution of caspase-8, a key cell death initiator molecule that was first identified in humans.

By performing the most extensive evolutionary analysis of the Casp8 protein to date, they found that Casp8 activity arose very early (more than 500 MYA), and is universally conserved throughout evolution, demonstrating its functional significance throughout the animal kingdom.

"It is of great significance that the programmed cell death system is established in more simpler animals," said professor Sakamaki.

In addition, they were able to substitute Casp8 proteins from non-mammalian examples and trigger the same cell death pathways when placed into cultured mammalian cell experiments using a killing assay, demonstrating its universal functionality in evolution. They also demonstrate that key protein interactions between Casp8 and another called FADD are also observed across the animal kingdom.

Thus, the cell death toolkit is of core importance to animal evolution, with cell death occurring to eliminate unnecessary, non-functional, unhealthy, or dangerous cells from the body. "In mammals, the cells producing a death ligand and expressing death receptor (and FADD/casp8) are different, suggesting that cell-cell communication is required for this vital phenomenon," said Sakamaki.

 


Story Source:

The above story is based on materials provided by Molecular Biology and Evolution (Oxford University Press). Note: Materials may be edited for content and length.


Journal Reference:

  1. K. Sakamaki, K. Shimizu, H. Iwata, K. Imai, Y. Satou, N. Funayama, M. Nozaki, M. Yajima, O. Nishimura, M. Higuchi, K. Chiba, M. Yoshimoto, H. Kimura, A. Y. Gracey, T. Shimizu, K. Tomii, O. Gotoh, K. Akasaka, T. Sawasaki, D. J. Miller. The Apoptotic Initiator Caspase-8: Its Functional Ubiquity and Genetic Diversity during Animal Evolution. Molecular Biology and Evolution, 2014; DOI: 10.1093/molbev/msu260

First step: From human cells to tissue-engineered esophagus

 


This images shows a tissue-engineered esophagus.

In a first step toward future human therapies, researchers at The Saban Research Institute of Children's Hospital Los Angeles have shown that esophageal tissue can be grown in vivo from both human and mouse cells. The study has been published online in the journal Tissue Engineering, Part A.

The tissue-engineered esophagus formed on a relatively simple biodegradable scaffold after the researchers transplanted mouse and human organ-specific stem/progenitor cells into a murine model, according to principal investigator Tracy C. Grikscheit, MD, of the Developmental Biology and Regenerative Medicine program of The Saban Research Institute and pediatric surgeon at Children's Hospital Los Angeles.

Progenitor cells have the ability to differentiate into specific types of cell, and can migrate to the tissue where they are needed. Their potential to differentiate depends on their type of "parent" stem cell and also on their niche. The tissue-engineering technique discovered by the CHLA researchers required only a simple polymer to deliver the cells, and multiple cellular groupings show the ability to generate a replacement organ with all cell layers and functions.

"We found that multiple combinations of cell populations allowed subsequent formation of engineered tissue. Different progenitor cells can find the right 'partner' cell in order to grow into specific esophageal cell types -- such as epithelium, muscle or nerve cells -- and without the need for exogenous growth factors. This means that successful tissue engineering of the esophagus is simpler than we previously thought," said Grikscheit.

She added that the study shows promise for one day applying the process in children who have been born with missing portions of the organ, which carries food, liquids and saliva from the mouth to the stomach. The process might also be used in patients who have had esophageal cancer -- the fastest growing type of cancer in the U.S. -- or otherwise damaged tissue, for example from accidentally swallowing caustic substances.

"We have demonstrated that a simple and versatile, biodegradable polymer is sufficient for the growth of tissue-engineered esophagus from human cells," added Grikscheit. "This not only serves as a potential source of tissue, but also a source of knowledge, as there are no other robust models available for studying esophageal stem cell dynamics. Understanding how these cells might behave in response to injury and how various donor cell types relate could expand the pool of potential donor cells for engineered tissue."

Additional contributors include first author Ryan G. Spurrier, MD, Allison L. Speer, MD, Xiaogang Hou, PhD and Wael N. El-Nachef, MD, of The Saban Research Institute of Children's Hospital Los Angeles. The study was supported by grants from the California Institute for Regenerative Medicine.


Story Source:

The above story is based on materials provided by Children's Hospital Los Angeles Saban Research Institute. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ryan Gregory Spurrier, Allison L Speer, Xiaogang Hou, Wael N El-Nachef, Tracy C Grikscheit. Murine and human tissue-engineered esophagus form from sufficient stem/progenitor cells and do not require microdesigned biomaterials. Tissue Engineering Part A, 2014; 141009005806008 DOI: 10.1089/ten.TEA.2014.0357