segunda-feira, 7 de julho de 2014

Expressões Idiomáticas Inglês-Português - 1

 

A Língua Inglesa possui algumas armadilhas para quem não a fala como língua materna, dentre elas estão as Expressões Idiomáticas (Idioms), que são figuras de linguagem onde um termo ou a frase assume um significado diferente do que as palavras teriam isoladamente. Assim, não basta saber o significado das palavras que formam a frase, é preciso olhar para todo o grupo de palavras que constitui a expressão para entender o seu significado. As Expressões Idiomáticas trazem conotações diferentes, que, na maioria das vezes, estão relacionadas às suas origens. É importante salientar que os idiomatismos não foram criados para serem armadilhas para os falantes estrangeiros, pelo contrário, elas tornam o Inglês Falado (Spoken English) mais natural. Relacionamos abaixo alguns exemplos de Expressões Idiomáticas mais usadas pelos falantes nativos da Língua Inglesa.

Act your age = Não seja infantil

All day long = O dia todo

Beyond a shadow of doubt = Sem sombra de dúvida

Blood is thicker than water = Os laços de família são mais fortes

Cross my heart = Juro por Deus
Everybody says so =Todos falam assim!

For goodness’ sake! = Pelo amor de Deus!

Good Lord! = Meu Deus!

Hand in Hand = De mãos dadas

I did quite well = Sai-me muito bem

Keep your eyes peeled = Fique atento

Leave it to me = Deixa comigo

Like hell! = Uma ova!

May I have the floor? = Posso falar?

Mum’s the word = Boca de siri

Never heard of = Nunca ouvi dizer

Never mind = Deixa prá lá / Não tem importância

Once and for all = De uma vez por todas

Pretty soon = Em breve

Quite a bit = muito, um montão, bastante, um bocado

Right over there = Logo ali

See you there = Até lá

Shoot the works = Manda brasa

Talk is cheap = Falar é fácil

Thank God = Graças a Deus

It is up to you = Você que sabe

You know best = Você é quem sabe

Take your time = Não se apresse

So far, so good? = Até aqui, tudo bem?

It is not your business, Mind your own business= Não é da sua conta, cuide da sua vida.

To kick the bucket = Bater as botas / Morrer

How come? = Como é que pode?

 

It is raining cats and dogs = Está chovendo muito

Over the moon = Estar feliz / Estar no mundo da lua de tão contente

On the crest of a wave = Estar por cima
To put the cat out of the bag = Contar um segredo / Não esconder o jogo

What's up? = E aí, como é que é? (informal)

Make yourself at home / ease / comfortable = Sinta-se em casa / Fique à vontade

Help yourself / Be my guest / Go ahead (informal) = Sirva-se

Let's keep in touch = Vamos manter contato

Look at/on the bright side = Veja o lado bom das coisas

Look here! = Escuta aqui!

Look lively! = Acorda! (pedindo atenção)

I will always be there for you = Sempre estarei ao seu lado

Good thinking = Bem pensado

To be in a bad/good mood = Estar de mau/bom humor

Snitcher = Dedo-duro

I think so = Acho que sim

I don't think so = Acho que não

Nothing ventured, nothing gained = Quem não arrisca, não petisca

No pain, no gain = Quem não arrisca não petisca

On second thought = Pensando bem

As far as I know... = Que eu saiba...

As good as it gets! = Melhor é impossível!

As if! = Até parece!

As lost as a nun on a honeymoon = Mais perdido que cego em tiroteio

At rock bottom = No fundo do poço / Estar por baixo

Fair game = Presa fácil

Fair play = Jogo limpo

And so what? = E daí?

A cat may look at a king = Olhar não tira pedaço

All in good time = Tudo a seu tempo

And I am a Dutchman = E eu acredito em Coelhinho da Páscoa / Papai Noel (para expressar descrença)

Anything goes! = Vale tudo!

To be a bad egg = Não ser flor que se cheire

Bite your tongue! = Vira essa boca pra lá!

Lies don't travel far = Mentira tem perna curta

Cheer down! = Menos, menos! (quando alguém exagera)

Clear the way! = Abram caminho!

Credit where credit is due = Verdade seja dita

Damned if you do, damned if you don't = Se correr o bicho pega, se ficar o bicho come

LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

 

Photo 07-07-2014 09 57 08

Mobile messaging apps may be reaching both saturation point and convergence, but that doesn’t mean there isn’t room for more, right? Meet LokLok.

LokLok sells itself as a connected lock-screen that is always in-sync with you and your favorite group of people. You can draw, write messages, or add photos to your screen, and it shows up on your friends’ screens – all without leaving the lock-screen. This could be a single person, or a whole group.

How it works

With LokLok set as your lockscreen, you tap twice to make it editable (i.e. you choose to doodle), or swipe up to unlock and use your phone as normal.

g 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

h 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

To draw, you simply use a single finger to make your markings, and to erase you pinch outwards with two fingers.

a3 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

b3 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

There’s a number of editing tools available including one that takes you directly to your camera or camera roll, while the palette option lets you choose your tool, brush-stroke and color. Next up, hit the three-dots at the bottom to share (or invite friends to join) and you’re good to go.

c3 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

f2 220x391 LokLok for Android lets you send doodles, photos and messages directly from your lockscreen

LokLok is effectively a synchronized whiteboard, making it as easy as possible to share notes, pictures and random doodles with anyone. Without anyone having to unlock their device.

LokLok says it’s looking to push push experiments around unconventional messaging one step further. “The idea is to use the first interaction that a user has with his phone for the communication process,” explains creator Guillermo Landin, who works as a UX director for Portugal-based Kwamecorp.

“I was curious to see if I could use the screen as the communication channel itself,” continues Guillermo. The original experiment developed into a prototype based on widgets and an HTML5 canvas, then Guillermo brought in a small team to work specifically on the app.

For the security conscious among you, LokLok only keeps the latest image of each group on the server, so when you clear the screen, it’s gone forever. Ephemeral is the key (buzz)word here.

On the surface, this seems like a classic example of something that’s possible on Android but not iOS. However, we’re told they’re already working on a version for Apple’s mobile operating system, “focusing on new possibilities that iOS 8 will introduce.”

New treatment for borderline personality disorder


A group of Swiss investigators reports on a new type of psychotherapy for borderline personality disorder in the current issue of Psychotherapy and Psychosomatics.

Motive-oriented therapeutic relationship (MOTR) was postulated to be a particularly helpful therapeutic ingredient in the early treatment phase of patients with personality disorders, in particular with borderline personality disorder (BPD).

This randomized controlled study using an add-on design is the first study to test this assumption in a 10-session general psychiatric treatment with patients presenting with BPD on symptom reduction and therapeutic alliance. A total of 85 patients were randomized. They were either allocated to a manual-based short variant of the general psychiatric management (GPM) treatment (in 10 sessions) or to the same treatment where MOTR was deliberately added to the treatment. Treatment attrition and integrity analyses yielded satisfactory results.

After performing the inter-to-treat analysis, results suggested a global efficacy of MOTR, in the sense of an additional reduction of general problems, i.e. symptoms, interpersonal and social problems. However, they also showed that MOTR did not yield an additional reduction of specific borderline symptoms. It was also shown that a stronger therapeutic alliance, as assessed by the therapist, developed in MOTR treatments compared to GPM.

These findings suggest that adding MOTR to psychiatric and psychotherapeutic treatments of BPD is promising. Moreover, the findings shed additional light on the perspective of shortening treatments for patients presenting with BPD.

Temperament may contribute to cardiac complications in high blood pressure


Temperament has been traditionally associated with high blood pressure. A new study that has appeared in the current issue of Psychotherapy and Psychosomatics has substantiated this issue.

Major depression and coronary heart disease have a strong, bidirectional relationship. A type A behavioral pattern, as well as cyclothymic disorder, is a subclinical manifestation of bipolar illness, and in cardiovascular patients may result in extreme behavioral changes detrimental to cardiac prognosis.

To further characterize this most vulnerable group, Authors examined the affective temperamental traits (Temperament Evaluation of Memphis, Pisa, Paris and San Diego Autoquestionnaire, TEMPS-A) on depressive, cyclothymic, hyperthymic, irritable and anxious subscales, ICD-10-diagnosed depression and depressive symptoms (Beck Depression Inventory, BDI) in relation to cardiac complications (CC) requiring acute hospitalization (acute coronary syndrome, acute myocardial infarction) in a primary hypertensive outpatient population.

Results showed that patients with CC scored markedly higher on the cyclothymic temperament scale (p = 0.027) than those without CC. Also, cyclothymic temperament significantly predicted CC independently of depression (either ICD-10-diagnosed or depressive symptoms), age, gender and smoking in hypertensive outpatients.

Even though, the study presents some limitations (cross-sectional nature hinders drawing a causal relationship, the relatively small sample size and low proportion of CC restrict generalizability), the findings shed light on the possible role of affective temperaments in cardiovascular morbidity and carry the advantage of exploring trait-like characteristics which precede and also determine the type of depression affecting the clinical outcome. Further research in the field would enrich the preventative options in clinical medicine in the future.


Story Source:

The above story is based on materials provided by Journal of Psychotherapy and Psychosomatics. Note: Materials may be edited for content and length.


Journal Reference:

  1. Ajandek Eory, Sandor Rozsa, Peter Torzsa, Laszlo Kalabay, Xenia Gonda, Zoltan Rihmer. Affective Temperaments Contribute to Cardiac Complications in Hypertension Independently of Depression. Psychotherapy and Psychosomatics, 2014; 83 (3): 187 DOI: 10.1159/000357364

Participant Index Seeks to Determine Why One Film Spurs Activism, While Others Falter

 

By MICHAEL CIEPLYJULY 6, 2014

 

The documentary film "The Square," about the Egyptian uprising in Tahrir Square, Cairo. On an impact scale of 100, the film scored an average of 92. Credit Noujaim Films

LOS ANGELES — You watched the wrenching documentary. You posted your outrage on Twitter. But are you good for more than a few easy keystrokes of hashtag activism?

Participant Media and some powerful partners need to know.

For the last year Participant, an activist entertainment company that delivers movies with a message, has been quietly working with the Bill & Melinda Gates Foundation, the Knight Foundation and the University of Southern California’s Annenberg School for Communication and Journalism to answer a question vexing those who would use media to change the world.

That is, what actually gets people moving? Do grant-supported media projects incite change, or are they simply an expensive way of preaching to the choir?

Ultimately, the answers may help determine which projects get financed, which formats are favored and how stories are structured. That could be true for so-called double bottom line companies like Participant, which seek to profit (or at least break even) while creating social change, and also for nonprofits like the Gates Foundation, which increasingly rely on entertainment-style media (like the education documentary “Waiting for ‘Superman’ ”) to drive an agenda.

 

"The Square" scored extremely high for emotional involvement at 97 out of 100, but dropped to 87 in terms of provoking action. Credit Netflix/Noujaim Films

More immediate, those behind the effort say, new measures of social impact will enable sharper focus and rapid course corrections in what have often been guesswork campaigns to convert films into effective motivational weaponry. That approach would apply to a hit like the movie “Lincoln,” which counseled civic engagement, or to a box-office miss like the antifracking film “Promised Land.” Both were Participant-backed films.

To get the answers it wants, Participant is developing a measuring tool that it calls the Participant Index, assisted in the effort by the Annenberg school’s Media Impact Project. In rough parallel to the Nielsen television ratings, the still-evolving index compiles raw audience numbers for issue-driven narrative films, documentaries, television programs and online short videos, along with measures of conventional and social media activity, including Twitter and Facebook presence.

The two measures are then matched with the results of an online survey, about 25 minutes long, that asks as many as 350 viewers of each project an escalating set of questions about their emotional response and level of engagement.

Did it affect you emotionally? Did you share information about it? Did you boycott a product or company? Did it change your life?

“If this existed, we would not be doing it,” said James G. Berk, chief executive of Participant. “We desperately need more and more information, to figure out if what we were doing is actually working.”

The answers result in a score that combines separate emotional and behavioral measures. On a scale of 100, for instance, “The Square,” a documentary about Egyptian political upheaval that was included in Participant’s first echelon of 35 indexed titles this year, scored extremely high for emotional involvement, with a 97, but lower in terms of provoking action, with an 87, for a combined average of 92.

By contrast, “Farmed and Dangerous,” a comic web series about industrial agriculture, hit 99 on the action scale, as respondents said, for instance, that they had bought or shunned a product, and 94 for emotion, for an average of 97. That marked it as having potentially higher impact than “The Square” among those who saw it.

 

The documentary "The Cove," which looks closely at dolphin killing in Japan, had worldwide ticket sales of just $1.2 million after its release in 2009. Yet it has repeatedly led to campaigns to protect the Japanese dolphins. Credit Oceanic Preservation Society/Roadside Attractions

Daniel Green, the deputy director for strategic media partnerships at the Gates Foundation, traces the new drive for impact measurement to a Seattle meeting in December 2011 among about two dozen representatives of nonprofits with an interest in social change.

“Grantors didn’t have a lot of sophistication around their analytics,” said Michael Maness of the Knight Journalism and Media Innovation program, a group that attended. He joined Mr. Green last month in describing frustration among nonprofits at their inability to gauge how much change their projects are prompting.

The Seattle gathering led to an association with the Annenberg school’s Norman Lear Center, which early last year established its Media Impact Project. That project then served as a consultant to Participant in creating its index, which received $4.2 million in combined financing from the Knight and Gates foundations and from George Soros’s Open Society Foundations.

The methodologies being used for the index will be provided on an open-source basis to those who are interested — whether on the left or right or in the center of the ideological spectrum.

“We’re developing a set of tools and measures that will be available for any researcher, no matter what their viewpoint,” said Martin Kaplan, director of the Lear Center.

Participant, created in 2004 by the eBay co-founder Jeffrey S. Skoll, is using that methodology to build a proprietary database. It will feature three echelons with 35 projects each, or about 100 distinct bits of media, annually.

The company will lean heavily toward films and television shows of its own, especially those carried on its activism-driven online and pay-television network, Pivot. But it will also index properties for partners, like the Gates and Kaiser Family foundations, and for companies or others who will pay a fee.

Photo

Participant was created in 2004 by the eBay co-founder Jeffrey S. Skoll, left, pictured here with James G. Berk, chief executive. Credit Emily Berl for The New York Times

(Prices have not been set, Mr. Berk said, but he expects to serve nonprofits at cost. He declined to say how much Participant has invested in the index.)

In an inaugural general survey, which polled 1,055 of its viewers in March and April of this year, Chad Boettcher, Participant’s executive vice president for social action, and Caty Borum Chattoo, a researcher and communications professor at American University, found some perhaps surprising results.

Even among the presumably progressive Participant audience, crime ranked near the top of the list of 40 primary concerns. It was cited by 73 percent of respondents as an important social issue, placing it just behind human rights, health care and education.

Gay rights, female empowerment and prison sentencing reform, by contrast, ranked near the bottom of the list, while climate change was stuck in the middle, a concern among 59 percent of respondents. Digital intellectual property issues, at 38 percent, brought up the rear.

Stories about animal rights and food production, it turned out, were the most likely to provoke individual action. But tales about economic inequality — not so much.

Over all, said Marc Karzen, a social media entrepreneur whose company, RelishMix, advises film and television marketers, Participant will most likely affirm what is becoming clear to conventional film studios: Impact can be less about persuasion than nudging an audience to go where it is already pointed.

You have to embrace your fans, not shout at them,” Mr. Karzen said. “They need to be inspired to spread the word.”

One of the weirdest problems in measuring social impact, and one still unresolved, Mr. Boettcher said, is the paradox of “The Cove.”

That documentary, which looks closely at dolphin killing in Japan, had worldwide ticket sales of just $1.2 million after its release in 2009. Yet it has repeatedly led to campaigns to protect the Japanese dolphins, Mr. Boettcher notes, particularly among activists who are aware of the film but will not watch (and hence, would not be counted under the current methodology of the index) because of its gory content.

“They don’t want to see it,” Mr. Boettcher said, “but they will sign up.”

A version of this article appears in print on July 7, 2014, on page B1 of the New York edition with the headline: Divining Why One Film Spurs Activism, While Others Falter. Order ReprintsToday's PaperSubscribe

Personality and heart attacks: A new look

 

A new study published in the current issue of Psychotherapy and Psychosomatics has addressed the relationship between personality and heart attacks. Distressed (type D) personality (TDP), characterized by high negative affectivity (NA) and social inhibition (SI), along with depression, anxiety and other negative affects (such as demoralization, hopelessness, pessimism and rumination) have been implicated as potential risk factors for coronary artery disease. While some evidence suggests that the NA dimension of TDP overlaps at least partially with depression, other studies underline how 'TDP refers to a chronic, more covert form of distress that is distinct from depression'.

In this study, authors aimed to clarify whether, among never depressed patients at their first acute coronary syndrome (ACS), there is an overlap between the constructs of TDP and depression, evaluating the stability of NA and SI 6 months after the ACS, and their relationship with depressive symptoms. Patients consecutively admitted to the Coronary Intensive Care Unit of the University Hospital of Parma between January 2009 and March 2012 who had their first ACS and no history of major depression (MD) or other psychiatric disorders, were included.

During the follow-up period 30 patients developed depressive symptoms (MD: n = 12; minor depression (md): n = 18), whereas 220 subjects maintained a nondepressive condition throughout the study period. At baseline the NA and SI levels were higher in subjects who developed depression than in patients who did not. However, at the baseline evaluation 19 patients without previous depressive episodes already satisfied the criteria for md. Interestingly, at baseline these subjects showed higher levels of NA and SI than subjects without md. Among patients who developed depression (n = 30) HADS scores significantly changed during the 6-month follow-up: both anxiety and depression scores increased from baseline to the second month of follow-up and then decreased. The same pattern of change was observed for the NA score, whereas the SI score did not vary during follow-up. In nondepressed patients, both HADS depression and anxiety scores and NA score significantly decreased throughout the follow-period, whereas the SI did not change.

In this study, the overlap between depressive psychopathology and NA features is suggested by the course of these two dimensions over time. Indeed, in both depressed and nondepressed patients, NA levels were not stable during the 6-month follow-up, but they changed along with the variation of HADS scores. This finding suggests that the NA dimension is sensitive to mood-state, because its levels increase and decrease according to the fluctuation of severity of depressive and anxious symptoms. This result supports the view that the disposition to experience and report negative emotions (NA) can be sensitive to mood-state. Therefore, the presence of depressive state is crucial when assessing TDP, since NA and anhedonic depression are partially overlapping and co-varying constructs.


Story Source:

The above story is based on materials provided by Journal of Psychotherapy and Psychosomatics. Note: Materials may be edited for content and length.


Journal Reference:

  1. Carlo Marchesi, Paolo Ossola, Francesca Scagnelli, Francesca Paglia, Sonja Aprile, Alberto Monici, Matteo Tonna, Giulio Conte, Franco Masini, Chiara De Panfilis, Diego Ardissino. Type D Personality in Never Depressed Patients at Their First Acute Coronary Syndrome. Psychotherapy and Psychosomatics, 2014; 83 (3): 190 DOI: 10.1159/000358525

domingo, 6 de julho de 2014

How you cope with stress may increase your risk for insomnia


A new study is the first to identify specific coping behaviors through which stress exposure leads to the development of insomnia.

Results show that coping with a stressful event through behavioral disengagement -- giving up on dealing with the stress -- or by using alcohol or drugs each significantly mediated the relationship between stress exposure and insomnia development. Surprisingly, the coping technique of self-distraction -- such as going to the movies or watching TV -- also was a significant mediator between stress and incident insomnia. Furthermore, the study found that cognitive intrusion -- recurrent thoughts about the stressor -- was a significant and key mediator, accounting for 69 percent of the total effect of stress exposure on insomnia.

"Our study is among the first to show that it's not the number of stressors, but your reaction to them that determines the likelihood of experiencing insomnia," said lead author Vivek Pillai, PhD, research fellow at the Sleep Disorders & Research Center at Henry Ford Hospital in Detroit, Michigan. "While a stressful event can lead to a bad night of sleep, it's what you do in response to stress that can be the difference between a few bad nights and chronic insomnia."

Study results are published in the July 1 issue of the journal Sleep.

The study involved a community-based sample of 2,892 good sleepers with no lifetime history of insomnia. At baseline the participants reported the number of stressful life events that they had experienced in the past year, such as a divorce, serious illness, major financial problem, or the death of a spouse. They also reported the perceived severity and duration of each stressful event. Questionnaires also measured levels of cognitive intrusion and identified coping strategies in which participants engaged in the seven days following the stressful event. A follow-up assessment after one year identified participants with insomnia disorder, which was defined as having symptoms of insomnia occurring at least three nights per week for a duration of one month or longer with associated daytime impairment or distress. "This study is an important reminder that stressful events and other major life changes often cause insomnia," said American Academy of Sleep Medicine President Dr. Timothy Morgenthaler. "If you are feeling overwhelmed by events in your life, talk to you doctor about strategies to reduce your stress level and improve your sleep."

According to the authors, the study identified potential targets for therapeutic interventions to improve coping responses to stress and reduce the risk of insomnia. In particular, they noted that mindfulness-based therapies have shown considerable promise in suppressing cognitive intrusion and improving sleep.

"Though we may not be able to control external events, we can reduce their burden by staying away from certain maladaptive behaviors," said Pillai.

The American Academy of Sleep Medicine reports that short-term insomnia disorder lasting less than three months occurs in 15 to 20 percent of adults and is more prevalent in women than in men.

The research was performed under the supervision of Thomas Roth, PhD, and Christopher Drake, PhD, in the Sleep & Research Center at Henry Ford Hospital in Detroit. The study was supported by funding from the National Institute of Mental Health (NIMH) of the National Institutes of Health (NIH).


Story Source:

The above story is based on materials provided by American Academy of Sleep Medicine. Note: Materials may be edited for content and length.


Journal Reference:

  1. Vivek Pillai, Thomas Roth, Heather M. Mullins, Christopher L. Drake. Moderators and Mediators of the Relationship Between Stress and Insomnia: Stressor Chronicity, Cognitive Intrusion, and Coping. SLEEP, 2014; DOI: 10.5665/sleep.3838

New insights on conditions for new blood vessel formation


A new capillary sprouting off a blood vessel.

Angiogenesis, the sprouting of new blood vessels from pre-existing ones, is essential to the body's development. As organs grow, vascular networks must grow with them to feed new cells and remove their waste. The same process, however, also plays a critical role in the onset and progression of many cancers, as it allows the rapid growth of tumors.

With lifesaving applications possible in both inhibiting and accelerating the creation of new blood vessels, a more fundamental understanding of what regulates angiogenesis is needed. Now, researchers at the University of Pennsylvania, Boston University and Harvard University have uncovered the existence of a threshold above which fluid flowing through blood vessel walls causes new capillaries to sprout.

This discovery could help pave the way for cancer-fighting drugs, treatments for the hardened blood vessels found in the cardiovascular disease arthrosclerosis or even growing synthetic organs in the lab.

The research was led by postdoctoral fellow Peter Galie of the Department of Bioengineering in Penn's School of Engineering and Applied Science and Christopher Chen, then a professor of bioengineering at Penn who is now at Boston University and an associate faculty member of the Wyss Institute for Biologically Inspired Engineering at Harvard University. They collaborated with Duc-Huy Nguyen, Colin Choi and Daniel Cohen, all members of Chen's lab, and professor Paul Janmey, also of the Department of Bioengineering, as well as the Department of Physiology in Penn's Perelman School of Medicine.

Their study was published in the Proceedings of the National Academy of Sciences.

The team's experiments incorporated "blood-vessel-on-a-chip" devices, which use microfluidic technology to simulate processes that normally occur deep within tissues. They found that cells lining each artificial vessel sprouted to form new vessels once the force exerted by fluid flow through the vessel exceeded a certain threshold.

"These findings suggest that our blood vessels can sense when blood flow exceeds their carrying capacity and respond by producing additional vessels on demand," Chen explained. "Perhaps we could one day take advantage of this response to enhance vessel regrowth where the need is critical, such as after a heart attack."

During their experiments, the researchers controlled the fluid flow within the artificial vessel, and ultimately where new vessels would sprout, by changing the shape and orientation of thin needles deployed within a collagen gel containing each vessel. Using a mathematical model, they predicted the exact spots along the vessel where force exceeded the sprouting threshold, thereby pinpointing the location where new vessels would form.

Now the researchers aim to advance new experiments designed to figure out how cells sense this mechanical threshold.

"The logical next step is to determine the molecular mechanism behind this phenomenon," said Galie, "what proteins are involved and how might they be targeted in new drug therapies."

Their work was funded by the National Institutes of Health and the University of Pennsylvania's Center for Engineering Cells and Regeneration, where Chen was the founding director.


Story Source:

The above story is based on materials provided by University of Pennsylvania. Note: Materials may be edited for content and length.


Journal Reference:

  1. P. A. Galie, D.-H. T. Nguyen, C. K. Choi, D. M. Cohen, P. A. Janmey, C. S. Chen. Fluid shear stress threshold regulates angiogenic sprouting. Proceedings of the National Academy of Sciences, 2014; 111 (22): 7968 DOI: 10.1073/pnas.1310842111

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Biological signal processing: Body cells -- instrumentalists in a symphony orchestra


Like an isolated note in a symphony orchestra, an isolated signal in the cell is of subordinate importance.

Every organism has one aim: to survive. Its body cells all work in concert to keep it alive. They do so through finely tuned means of communication. Together with cooperation partners from Berlin and Cambridge, scientists at the Luxembourg Centre for Systems Biomedicine (LCSB) of the University of Luxembourg have now successfully revealed for the first time the laws by which cells translate signals from their surroundings into internal signals. Like an isolated note in a symphony orchestra, an isolated signal in the cell is of subordinate importance. "What is important is the relative variation of intensity and frequency at which the signals are transmitted from the cell membrane into the cell," says Dr. Alexander Skupin, who led the studies at LCSB. The research group published their results now in the scientific journal Science Signaling.

The instruments in an orchestra produce signals -- musical notes -- by causing the air to vibrate. Inside a cell, calcium ions carry signals. When a piece of information from the environment -- say a biological messenger -- meets the outer envelope of the cell, calcium ions are released inside the cell. There, they control various adaptation processes. "At first sight, there is no simple pattern to the ion impulses," Skupin explains; "yet they still culminate in a meaningful response inside the cell, like the activation of a specific gene, for instance."

In order to determine the laws underlying this phenomenon, the researchers studied human kidney cells and rat liver cells using a combination of imaging technologies and mathematical methods. They discovered that the intensity and frequency of calcium impulses undergo extreme variation -- both cell-internally and cell-to-cell. Accordingly, the information they convey cannot be interpreted by analyzing isolated signals alone. "It's like in an orchestra, where studying an isolated note on its own allows no inference of the melody," Skupin continues the musical analogy. "You have to hear how the frequency and volume of all instruments vary and produce the melody. Then you gain an impression of the musical piece."

Now, for the first time, the researchers have managed to gain such an impression of the whole by listening in on the cells' communications. They discovered that the plethora of calcium impulses vary relatively to one another in a specific relationship: A stimulus from outside does not lead to an absolute increase in calcium impulses, but instead to a change in the frequency at which they occur -- in the concert hall, the notes of the instruments rise and fall in symphony. "This pattern is the actual signal that leads to a response in the cells," Skupin says. "With our analyses, we have rendered it interpretable."

"The results are of great importance for analyzing diseases," says Director of LCSB Prof. Dr. Rudi Balling. "We know that, in Parkinson's disease, the calcium balance in the nerve cells is disrupted, and suspect that errant communications between the cells could play a role in the onset of neurodegenerative diseases. With the discovery of the fundamental laws of these communications, as Alexander Skupin, his team and our cooperation partners have now achieved, we are set to take a major step forward in the analysis of Parkinson's disease."


Story Source:

The above story is based on materials provided by University of Luxembourg. Note: Materials may be edited for content and length.


Journal Reference:

  1. K. Thurley, S. C. Tovey, G. Moenke, V. L. Prince, A. Meena, A. P. Thomas, A. Skupin, C. W. Taylor, M. Falcke. Reliable Encoding of Stimulus Intensities Within Random Sequences of Intracellular Ca2 Spikes. Science Signaling, 2014; 7 (331): ra59 DOI: 10.1126/scisignal.2005237