terça-feira, 21 de outubro de 2014

Digest this: Cure for cancer may live in our intestines

 


The discovery of Robo1 protein in the intestinal stem cells (depicted in yellow) leads to tolerance of higher doses of chemoradiation for cancer patients.

Treating a cancerous tumor is like watering a houseplant with a fire hose -- too much water kills the plant, just as too much chemotherapy and radiation kills the patient before it kills the tumor.

However, if the patient's gastrointestinal tract remains healthy and functioning, the patient's chances of survival increase exponentially, said Jian-Guo Geng, associate professor at the University of Michigan School of Dentistry. Recently, Geng's lab discovered a biological mechanism that preserves the gastrointestinal tracts in mice who were delivered lethal doses of chemotherapy.

The findings, which will appear in the journal Nature, could revolutionize cancer therapy, Geng said.

"It's our belief that this could eventually cure later-staged metastasized cancer. People will not die from cancer, if our prediction is true," said Geng, who emphasized that the findings had not yet been proven in humans. "All tumors from different tissues and organs can be killed by high doses of chemotherapy and radiation, but the current challenge for treating the later-staged metastasized cancer is that you actually kill the patient before you kill the tumor.

"Now you have a way to make a patient tolerate to lethal doses of chemotherapy and radiotherapy. In this way, the later-staged, metastasized cancer can be eradicated by increased doses of chemotherapy and radiation."

Geng's lab found that when certain proteins bind with a specific molecule on intestinal stem cells, it revs intestinal stem cells into overdrive for intestinal regeneration and repair. Stem cells naturally heal damaged organs and tissues, but so-called "normal" amounts of stem cells in the intestine simply cannot keep up with the wreckage left behind by the lethal doses of chemotherapy and radiation required to successfully treat late-stage tumors.

However, the phalanx of extra stem cells protect the intestine and gastrointestinal tract, which means the patient can ingest nutrients, the body can perform other critical functions and the bacterial toxins in the intestine are prevented from entering the blood circulation, Geng said.

These factors could give the patient just enough of an extra edge to survive the stronger doses of chemotherapy and radiation, until the tumor or tumors are eradicated.

In the study, 50-to-75 percent of the mice treated with the molecule survived otherwise lethal doses of chemotherapy. All of the mice that did not receive the molecule died, Geng said.

"If you can keep the gut going, you can keep the patient going longer," Geng said. "Now we have found a way to protect the intestine. The next step is to aim for a 100-percent survival rate in mice who are injected with the molecules and receive lethal doses of chemotherapy and radiation."

Geng's lab has worked with these molecules, called R-spondin1 and Slit2, for more than a decade. These molecules repair tissue in combination with intestinal stem cells residing in the adult intestine.


Story Source:

The above story is based on materials provided by University of Michigan. Note: Materials may be edited for content and length.


Journal Reference:

  1. Wei-Jie Zhou, Zhen H. Geng, Jason R. Spence & Jian-Guo Geng. Induction of intestinal stem cells by R-spondin 1 and Slit2 augments chemoradioprotection. Nature, 2013 DOI: 10.1038/nature12416

 

Lab-developed intestinal organoids form mature human tissue in mice

 

October 19, 2014

Cincinnati Children's Hospital Medical Center

Researchers have successfully transplanted 'organoids' of functioning human intestinal tissue grown from pluripotent stem cells in a lab dish into mice -- creating an unprecedented model for studying diseases of the intestine. Scientists said that, through additional translational research, the findings could eventually lead to bioengineering personalized human intestinal tissue to treat gastrointestinal diseases.


Lab mouse (stock image). Researchers have successfully transplanted "organoids" of functioning human intestinal tissue grown from pluripotent stem cells in a lab dish into mice -- creating an unprecedented model for studying diseases of the intestine.

Researchers have successfully transplanted "organoids" of functioning human intestinal tissue grown from pluripotent stem cells in a lab dish into mice -- creating an unprecedented model for studying diseases of the intestine.

Reporting their results Oct. 19 online in Nature Medicine, scientists from Cincinnati Children's Hospital Medical Center said that, through additional translational research the findings could eventually lead to bioengineering personalized human intestinal tissue to treat gastrointestinal diseases.

"These studies support the concept that patient-specific cells can be used to grow intestine," said Michael Helmrath, MD, MS, lead investigator and surgical director of the Intestinal Rehabilitation Program at Cincinnati Children's. "This provides a new way to study the many diseases and conditions that can cause intestinal failure, from genetic disorders appearing at birth to conditions that strike later in life, such as cancer and Crohn's disease. These studies also advance the longer-term goal of growing tissues that can replace damaged human intestine."

The scientists used induced pluripotent stem cells (iPSCs) -- which can become any tissue type in the body -- to generate the intestinal organoids. The team converted adult cells drawn from skin and blood samples into "blank" iPSCs, then placed the stem cells into a specific molecular cocktail so they would form intestinal organoids.

The human organoids were then engrafted into the capsule of the kidney of a mouse, providing a necessary blood supply that allowed the organoid cells to grow into fully mature human intestinal tissue. The researchers noted that this step represents a major sign of progress for a line of regenerative medicine that scientists worldwide have been working for several years to develop.

Mice used in the study were genetically engineered so their immune systems would accept the introduction of human tissues. The grafting procedure required delicate surgery at a microscopic level, according to researchers. But once attached to a mouse's kidney, the study found that the cells grow and multiply on their own. Each mouse in the study produced significant amounts of fully functional, fully human intestine.

"The mucosal lining contains all the differentiated cells and continuously renews itself by proliferation of intestinal stem cells. In addition, the mucosa develops both absorptive and digestive ability that was not evident in the culture dish," Helmrath said. "Importantly, the muscle layers of the intestine also develop."

What This Means for Patients

The new findings eventually could be good news for people born with genetic defects affecting their digestive systems or people who have lost intestinal function from cancer, as well as Crohn's disease and other related inflammatory bowel diseases (IBD).

One of the advantages of using tissue generated from iPSCs is that the treatment process would involve the patient's own tissue, thus eliminating the risk and expense of life-long medications to prevent transplant rejection.

However, the researchers cautioned that it will take years of further research to translate lab-grown tissue replacement into medical practice. In the meantime, the discovery could have other, more immediate benefits by accelerating drug development and the concept of personalized medicine.

The current process for developing new medications depends on a long and imperfect process of animal testing. Promising compounds from the lab are tested in animals bred to mimic human diseases and conditions. Many compounds that prove effective and safe in mice turn out to be unsuccessful in human clinical trials. Others have mixed results, where some groups of patients clearly benefit from the new drug, but others suffer harmful side effects.

Lab-grown organoids have the potential to replace much of the animal testing stage by allowing early drug research to occur directly upon human tissue. Going straight to human tissue testing could shave years off the drug development process, researchers said.

The current study in Nature represents the latest step in years of stem cell and organoid research at Cincinnati Children's, much of which has been led by James Wells, PhD, and Noah Shroyer, PhD. Wells is a scientist in the divisions of Developmental Biology and Endocrinology at Cincinnati Children's and director of the Pluripotent Stem Cell Center. Shroyer is a scientist in the divisions of Gastroenterology, Hepatology & Nutrition and Developmental Biology.

Wells and colleagues first reported success at growing intestinal organoids in the lab in December 2010. Since then, the team has reported similar success at growing organoids of stomach tissue.


Story Source:

The above story is based on materials provided by Cincinnati Children's Hospital Medical Center. Note: Materials may be edited for content and length.


 

segunda-feira, 20 de outubro de 2014

Um bate-papo ao cair da noite - V

 

Esta semana li um banner em um importante website dos Estados Unidos, mais ou menos isto, não me lembro exatamente. “O que o Brasil precisa para mudar”…. Como isso é um tema que diz respeito à todos os países do mundo, ou quase todos, eu acho que somos muito queridos pelos nossos irmãos de outros países, e eu como brasileiro nato, fico até emocionado. (Maria, por favor, traga-me aquele lencinho de enxugar lágrimas…)

Bem, como mencionei acima, poucos países no mundo, estão tranquilos do ponto de vista econômico e social.  A economia mundial, e os valores morais da sociedade, hoje estão intimamente ligados. Um país avança em todos os sentidos quando o Mundo dá sinais de melhoria. O mundo é uma imensa máquina, e os países são as engrenagens dela. Qualquer problema numa engrenagem importante da máquina, e tudo fica meio emperrado.

Interessa muito ao mundo que o Brasil, (um país importante no cenário internacional, o 5º em área territorial, um dos maiores em população, GDP entre os maiores do mundo)… deslanche econômicamente, afinal somos um páis-alvo para as grandes empresas dos países (quase) desenvolvidos, e temos um grande contingente de consumidores, ávidos consumidores. Mas a máquina mundial está no momento meio emperrada….e isso nos afeta. Alguma ou algumas engrenagens estão precisando de ajustes, lubrificação….e isso nos afeta…e afeta a todos. Nada melhor do que um Simpósio Internacional para discutir isso… Parece que “Simpósio” não é o termo correto…Talvez “Conferência”, Reunião….

Mas, precisamos de um suporte mais efetivo dessas potências econômicas…. que pode ser realizado de várias maneiras…. (Transferência de tecnologias, por exemplo….) Ou aumento nas importações de produtos brasileiros…..

O que o Brasil precisa para mudar… Precisamos que o mundo mude junto conosco…. 

José S de Melo

Something about Belgium

 

Belgium  Dutch: België; French: Belgique; German: Belgien), officially the Kingdom of Belgium, is a federal monarchy in Western Europe. It is a founding member of the European Union and hosts the EU's headquarters as well as those of several other major international organisations such as NATO.[nb 1] Belgium covers an area of 30,528 square kilometres (11,787 sq mi) and has a population of about 11 million people.

Straddling the cultural boundary between Germanic and Latin Europe, Belgium is home to two main linguistic groups: the Dutch-speaking, mostly Flemish community, which constitutes about 59% of the population, and the French-speaking, mostly Walloon population and Brussels inhabitants, which comprises 41% of all Belgians. Additionally, there is a small group of German-speakers who are officially recognized.

Belgium's two largest regions are the Dutch-speaking region of Flanders in the north and the French-speaking southern region of Wallonia. The Brussels-Capital Region, officially bilingual, is a mostly French-speaking enclave within the Flemish Region. A German-speaking Community exists in eastern Wallonia. Belgium's linguistic diversity and related political conflicts are reflected in its political history and complex system of government.

Historically, Belgium, the Netherlands, and Luxembourg were known as the Low Countries; it once covered a somewhat larger area than the current Benelux group of states. The region was called Belgica in Latin, after the Roman province of Gallia Belgica, which covered more or less the same area. From the end of the Middle Ages until the 17th century, the area of Belgium was a prosperous and cosmopolitan centre of commerce and culture. From the 16th century until the Belgian Revolution in 1830, when Belgium seceded from the Netherlands, the area of Belgium served as the battleground between many European powers, causing it to be dubbed the "Battlefield of Europe," a reputation strengthened by both World Wars.

Upon its independence, Belgium participated in the Industrial Revolution and, during the course of the 20th century, possessed a number of colonies in Africa.The second half of the 20th century was marked by rising tensions between the Dutch-speaking and the French-speaking citizens fueled by differences in language and the unequal economic development of Flanders and Wallonia. This continuing antagonism has led to several far-reaching reforms, resulting in a transition from a unitary to a federal arrangement during the period from 1970 to 1993. Despite the reforms, tensions between the groups remain; the formation of a coalition government took 18 months following the June 2010 federal election.

 

Bruegge_huidenvettersplein

Bruegge Huidenvettersplein

 

Liege_View

Liege View

Hubble finds extremely distant galaxy through cosmic magnifying glass

 


The heart of the mammoth galaxy cluster Abell 2744, also known as Pandora's Cluster, is shown in this Hubble Space Telescope image. The cluster is so massive that its powerful gravity bends the light from galaxies far behind it, making background objects appear larger and brighter in a phenomenon called gravitational lensing. These powerful lenses allow astronomers to find many dim, distant structures that otherwise might be too faint to see. The small white boxes, labeled "a," "b," and "c," mark multiple images from the same background galaxy, one of the farthest, faintest, and smallest galaxies ever seen. The diminutive object is estimated to be over 13 billion light-years away. Enlarged views of the multiple images are shown in the insets at right.

Peering through a giant cosmic magnifying glass, NASA's Hubble Space Telescope has spotted one of the farthest, faintest, and smallest galaxies ever seen. The diminutive object is estimated to be over 13 billion light-years away.

This new detection is considered one of the most reliable distance measurements of a galaxy that existed in the early universe, said the Hubble researchers. They used two independent methods to estimate its distance.

The galaxy appears as a tiny blob that is only a small fraction of the size of our Milky Way galaxy. But it offers a peek back into a time when the universe was only about 500 million years old, roughly 3 percent of its current age of 13.7 billion years. Astronomers have uncovered about 10 other galaxy candidates at this early era. But this newly found galaxy is significantly smaller and fainter than most of those other remote objects detected to date.

"This object is a unique example of what is suspected to be an abundant, underlying population of extremely small and faint galaxies at about 500 million years after the big bang," explained study leader Adi Zitrin of the California Institute of Technology in Pasadena. "The discovery is telling us that galaxies as faint as this one exist, and we should continue looking for them and even fainter objects so that we can understand how galaxies and the universe have evolved over time."

The galaxy was detected as part of the Frontier Fields program, an ambitious three-year effort, begun in 2013, that teams Hubble with NASA's other Great Observatories -- the Spitzer Space Telescope and the Chandra X-ray Observatory -- to probe the early universe by studying large galaxy clusters. These clusters are so massive that their gravity deflects light passing through them, magnifying, brightening, and distorting background objects in a phenomenon called gravitational lensing. These powerful lenses allow astronomers to find many dim, distant structures that otherwise might be too faint to see.

In this new discovery, the lensing power of the mammoth galaxy cluster Abell 2744, nicknamed Pandora's Cluster, produced three magnified images of the same galaxy. Each magnified image makes the galaxy appear as much as 10 times larger and brighter than it would look without the intervening lens.

An analysis of the distant galaxy shows that it measures merely 850 light-years across, 500 times smaller than the Milky Way, and is estimated to have a mass of only 40 million suns. The galaxy's star formation rate is about one star every three years (one-third the star formation rate in the Milky Way). Although this may seem low, Zitrin said that given its small size and low mass, the tiny galaxy is in fact rapidly evolving and efficiently forming stars.

"Galaxies such as this one are probably small clumps of matter that are starting to form stars and shine light, but they don't have a defined structure yet," Zitrin said. "Therefore, it's possible that we only see one bright clump magnified due to the lensing, and this is one possibility as to why it is smaller than typical field galaxies of that time." Zitrin's team spotted the galaxy's gravitationally multiplied images using near-infrared and visible-light photos of the galaxy cluster taken by Hubble's Wide Field Camera 3 and Advanced Camera for Surveys. But at first they didn't know how far away it was from Earth.

Normally, astronomers use spectroscopy to determine an object's distance. The farther away a galaxy, the more its light has been stretched by the universe's expansion. Astronomers can precisely measure this effect through spectroscopy, which characterizes an object's light.

But the gravitationally lensed galaxy and other objects found at this early epoch are too far away and too dim for astronomers to use spectroscopy. Astronomers instead analyze an object's color to estimate its distance. The universe's expansion reddens an object's color in predictable ways, which scientists can measure.

Members of Zitrin's team not only performed the color-analysis technique, but they also took advantage of the multiple images produced by the gravitational lens to independently confirm their distance estimate. The astronomers measured the angular separation between the three magnified images of the galaxy in the Hubble photos. The greater the angular separation due to lensing, the farther away the object is from Earth. To test this concept, the astronomers compared the three magnified images with the locations of several other multiply imaged objects lensed by Abell 2744 that are not as far behind the cluster. The angular distance between the magnified images of the closer galaxies was smaller.

"These measurements imply that, given the large angular separation between the three images of our background galaxy, the object must lie very far away," Zitrin explained. "It also matches the distance estimate we calculated, based on the color-analysis technique. So we are about 95 percent confident that this object is at a remote distance, at redshift 10 (a measure of the stretching of space since the big bang). The lensing takes away any doubt that this might be a heavily reddened, nearby object masquerading as a far more distant object."

Astronomers have long debated whether such early galaxies could have provided enough radiation to warm the hydrogen that cooled soon after the big bang. This process, called "reionization," is thought to have occurred 200 million to 1 billion years after the birth of the universe. Reionization made the universe transparent to light, allowing astronomers to look far back into time without running into a "fog" of cold hydrogen.

"We tend to assume that galaxies ionized the universe with their ultraviolet light," Zitrin said. "But we do not see enough galaxies or light that could do that. So we need to look at fainter and fainter galaxies, and the Frontier Fields and galaxy cluster lensing can help us achieve this goal."


Story Source:

The above story is based on materials provided by Space Telescope Science Institute (STScI). Note: Materials may be edited for content and length.


Journal Reference:

  1. Adi Zitrin, Wei Zheng, Tom Broadhurst, John Moustakas, Daniel Lam, Xinwen Shu, Xingxing Huang, Jose M. Diego, Holland Ford, Jeremy Lim, Franz E. Bauer, Leopoldo Infante, Daniel D. Kelson, Alberto Molino. A GEOMETRICALLY SUPPORTEDz∼ 10 CANDIDATE MULTIPLY IMAGED BY THE HUBBLE FRONTIER FIELDS CLUSTER A2744. The Astrophysical Journal, 2014; 793 (1): L12 DOI: 10.1088/2041-8205/793/1/L12

 

The first Indian satellite

 

Snap 2014-10-19 at 04.40.26

Interesting

 

 

Snap 2014-10-19 at 04.52.57

Será que ela perdoou?

Snap 2014-10-19 at 04.51.14

Vejam como as mamães sofrem.

Snap 2014-10-19 at 04.49.47

Which couple has won the dance marathon?

Snap 2014-10-19 at 04.48.12